What is a discordant alignment?

By default, bowtie2 looks for discordant alignments if it cannot find any concordant alignments.
Concordant alignments are those which satisfy the mapping constraints.
Discordant alignments are those which don't. But they are still uniquely mapped.

New phishing email targeting Caltech students: Login Error Detected IN0006782

This type of email again comes from a random email address with another email address as sender name to look like system administrator. A suspicious link provided and a strange department name(IT Service Alert Desk) they created added to the ridiculousness of this type of email.

-------------------Below is the original email, don't click on the link ----------------

noreply@caltech.edu b.m.brown-15@student.lboro.ac.uk via caltech.onmicrosoft.com	1:52 PM (4 minutes ago)
to

Your account has been suspended Log-On to recover your account

The Information Technology department encourages you to take the following measures to protect your account.

Sincerely,
IT Service Alert Desk

How did Nextera kit specifically generate hetero-products out of PCR reactions? Why do the products have two different adaptors on their two ends?

Suppression PCR

http://www.nature.com/nmeth/journal/v6/n11/pdf/nmeth.f.272.pdf

[Solved] MacBook doesn't recognize Kindle

You might be using a charging-only cable instead of a data cable.

reference: https://discussions.apple.com/thread/6691745?tstart=0

[兔兔助手] tutuapp new update: where is the newer version of Pokemon GO 懒人版

1. Download 兔兔助手正版
2. download from 兔兔助手 the app "Alfa Tournament"

Enjoy!


--------------

Now it's switched back ,  20160904

[Pokemon GO] Did I catch something rare? Pokemon rarity distribution and frequency counts

Everyone knows that a pidgey can be found everywhere while a charizard is not so common. But what's a more quantitative way to see how lucky you are when you see, say, an Abra or Haunter?

Here are the big data results (averaging all the places over the world, including coastal and non-coastal):

1. Overall, the rarity distribution is very uneven:
1.1 You catch a pidgey for every eight pokemons you come across
1.2 The top 9 abundant pokemons make up half of all the pokemons you see. In other words, half of your effort is actually dealing with the 9 most annoying pokemons you're tired of.

2. These are the detailed percentages:
     Based on their rarity, I divide them into 6 levels, with the most abundant group as Level 1 and the rarest group as Level 6. Below are the charts:

Level 6: those off the list (because our monitor cannot detect them frequent enough to estimate their probability)


Here is the full list with percentages of occurrence among all the pokemons. Ctrl + F / Command + F to find your favorite pokemon's score!

Pidgey	12.9%
Rattata	9.2%
Zubat	7.0%
Magikarp	5.5%
Weedle	4.8%
Spearow	4.3%
Ekans	2.78%
Psyduck	2.74%
Drowzee	2.61%
Caterpie	2.42%
Goldeen	2.13%
Paras	2.05%
Poliwag	2.04%
Eevee	2.03%
NidoranF	1.98%
Krabby	1.93%
Staryu	1.62%
NidoranM	1.61%
Venonat	1.55%
Meowth	1.27%
Mankey	1.19%
Tentacool	1.13%
Geodude	1.11%
Horsea	1.11%
Growlithe	1.09%
Sandshrew	1.06%
Clefairy	1.04%
Slowpoke	0.886%
Bellsprout	0.830%
Exeggcute	0.806%
Pinsir	0.797%
Oddish	0.778%
Pidgeotto	0.774%
Gastly	0.736%
Squirtle	0.731%
Magnemite	0.652%
Bulbasaur	0.642%
Cubone	0.595%
Rhyhorn	0.586%
Machop	0.567%
Shellder	0.525%
Golbat	0.516%
Voltorb	0.511%
Jigglypuff	0.474%
Doduo	0.464%
Diglett	0.445%
Abra	0.431%
Ponyta	0.389%
Jynx	0.384%
Dratini	0.366%
Kakuna	0.328%
Raticate	0.295%
Pikachu	0.286%
Tauros	0.281%
Charmander	0.277%
Seel	0.267%
Scyther	0.239%
Vulpix	0.230%
Koffing	0.192%
Metapod	0.183%
Tangela	0.178%
Omanyte	0.164%
Dodrio	0.155%
Poliwhirl	0.136%
Kabuto	0.131%
Arbok	0.122%
Onix	0.122%
Electabuzz	0.117%
Magmar	0.117%
Fearow	0.113%
Tentacruel	0.108%
Hypno	0.103%
Pidgeot	0.098%
Sandslash	0.094%
Golduck	0.089%
Nidorina	0.080%
Seaking	0.070%
Weepinbell	0.070%
Gloom	0.066%
Kingler	0.066%
Venomoth	0.066%
Graveler	0.061%
Nidorino	0.061%
Grimer	0.052%
Ivysaur	0.052%
Parasect	0.047%
Primeape	0.047%
Slowbro	0.047%
Seadra	0.038%
Arcanine	0.033%
Beedrill	0.033%
Butterfree	0.033%
Dragonair	0.033%
Kadabra	0.033%
Cloyster	0.028%
Haunter	0.028%
Hitmonlee	0.028%
Marowak	0.028%
Nidoking	0.023%
Persian	0.023%
Victreebel	0.023%
Wartortle	0.023%
Dewgong	0.019%
Electrode	0.019%
Flareon	0.019%
Hitmonchan	0.019%
Porygon	0.019%
Rhydon	0.019%
Snorlax	0.019%
Gengar	0.014%
Lickitung	0.014%
Magneton	0.014%
Mr.Mime	0.014%
Poliwrath	0.014%
Vaporeon	0.014%
Weezing	0.014%
Aerodactyl	0.009%
Charmeleon	0.009%
Clefable	0.009%
Dugtrio	0.009%
Golem	0.009%
Jolteon	0.009%
Lapras	0.009%
Rapidash	0.009%
Starmie	0.009%
Venusaur	0.009%
Wigglytuff	0.009%
Alakazam	0.005%
Exeggutor	0.005%
Farfetch'd	0.005%
Gyarados	0.005%
Ninetales	0.005%
Omastar	0.005%
Vileplume	0.005%

[solved] mysterious payment/credit card fraud from showpmt.com. what's that charge and how to cancel

If you noticed that your credit card activity history has something mentioning showpmt.com like these people, you'd be careful:

I was charged a total of $39.99 from my checking account for no apparent reason by CHECKCARD 02/11 SHOWPMT.COM 8772151910

posted 02/12/2014 by Joshua Montecino

                      

Usually the payment is under $100 but still it's a fraud, and a possible cause is you registered for some membership and provided them with your credit card information including the three digit number you have on the back of your card.

Solution:

1.Go to Showpmt.com

2.click membership ( manage your membership)

3. type your partial credit card information and cancel

Usually these should be enough

But to be safe you'd better request a replacement of your credit card by calling your credit card company

Tricks you should learn:

When asked for credit card information, never provide full information. If they ask you for full information, be alerted, and provide WRONG expire data or 3-digit code. If they are not legal chargers, they wouldn't know that your information provided was wrong. (I've tested some websites' fraud credit card signup by typing incorrect expire date. Registration was successful and my credit card was not charged.)

Take care

[兔兔助手tutuapp][solved] unverified app

Previously you could just follow the steps described here:
http://troubleshoo.blogspot.com/2016/08/ios9-untrusted-enterprise-developer.html

But 兔兔助手changed their company!!!
So you basically have to reinstall and retrust. That's it

Illumina Sequencing: Single indexing v.s. Dual indexing

http://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/indexed-sequencing-overview-guide-15057455-02.pdf

[Solved]Spotify cannot install on windows 8

1. Download the newest version of spotify here (if you are in the US) https://www.spotify.com/us/download/

2. press alt + ctrl + delete to open task manager

3. there should be two processes running by spotify. End them two by clicking on them and end task

4. Now go to folder C:\Users\Brian\AppData\Roaming
    and delete the Spotify folder to remove data

5. Install SpotifySetup.exe as usual

how to check your current ios version

settings -> general -> about

Scroll down and see "version"

[solved][Pokemon GO] where to find Mr. Mime, Kangaskhan and FARFETCH'D

Mr. Mime's home town is Madrid, Spain (Not London nor Paris nor Barcelona)
Kangaskhan is, of course, Australian, probably from Woollahra
Farfetch'd comes from Tsim Sha Tsui, Hong Kong

[Solved][Pokemon GO 懶人版] if you can't move and "GPS signal not found"

You can't move, you can see pokemons and you see the red bar with error messages "GPS signal not found", one solution is:

Turn off the wifi and use 4G or LTE

[Solved][Pokemon GO] No pokemons showing up, gyms and pokestops shown but not functional

If no pokemons are showing up where they should according to Skiplagged/Pokevision, and you can't flip any blue pokestops although you see them, one solution is:


Turn on the wifi if there is

[solved][pokemon GO] no poke stops or gyms or pokemons shown

The solution that worked for me:

restart the app again and again.

[Solved][Pokemon GO]our servers are humbled by your incredible response

My solution:
I turned off my wifi and started using LTE and it worked on my iphone5. It worked multiple times.
Apparently this error message is not necessarily related to their servers.

iOS9 Untrusted Enterprise Developer with no option to trust

Take pokemon GO hacked version as an example:

In iOS 9.1 and lower, go to Settings - General - Profiles - Your app source/Carey London Limited - tap on Trust button.

In iOS 9.2+, go to Settings - General - Device Management - Your app source/Carey London Limited - tap on Trust button.

reference: http://stackoverflow.com/questions/30722883/ios9-untrusted-enterprise-developer-with-no-option-to-trust

[chase freedom]how to verify receipt of replacement credit card

1. go to chase.com/verify card
2. log in
3. click the verify button