Extract genomic DNA from human sperm cells

1. collect fresh human semen. It can be stored at room temperature for at least one day
2. transfer human semen into eppendorf tubes and spin down at 20,000g 5min
3. carefully remove as much supernatant as possible without disturbing the pellet, add back PBS to adjust the volume, spin again
4. remove the supernatant
5. Add 200ul PBS and vortex
6. Add 200 ul ATL buffer from Qiagen DNeasy Blood & Tissue Kit, and 20ul proteinase K, vortex
7. Incubate 2 hours at 56 C
8. add 200 ul ethanol, vortex
9. transfer to Qiagen DNeasy column and spin at 6000g for 5min or until all the liquid passes through the column
10. discard the collection tube and flow-through, add 500ul of AW1 and spin at 6000g for 5min
11. discard the collection tube and flow-through, add 500ul of AW2 and spin at 6000g for 5min
12. discard the collection tube and flow-through and spin at 6000g for 5min to dry the column
13. use a 20ul pipette tip to remove the residue liquids on the top and bottom rings of the column
14. discard the collection tube, transfer to DNase-free eppendorf tubes add 200ul buffer AE to the dried column and incubate 1min at room temperature
15. spin at 6000g for 1 min and save the genomic DNA
You can use Nanodrop to measure, I can get 5ug genomic DNA per 1ml semen per Column