By default, bowtie2 looks for discordant alignments if it cannot find any concordant alignments.
Concordant alignments are those which satisfy the mapping constraints.
Discordant alignments are those which don't. But they are still uniquely mapped.
Wednesday, August 31, 2016
Sunday, August 28, 2016
New phishing email targeting Caltech students: Login Error Detected IN0006782
This type of email again comes from a random email address with another email address as sender name to look like system administrator. A suspicious link provided and a strange department name(IT Service Alert Desk) they created added to the ridiculousness of this type of email.
-------------------Below is the original email, don't click on the link ----------------
|
1:52 PM (4 minutes ago)
| |||
|
Your account has been suspended Log-On to recover your account
The Information Technology department encourages you to take the following measures to protect your account.
Sincerely,
IT Service Alert Desk
The Information Technology department encourages you to take the following measures to protect your account.
Sincerely,
IT Service Alert Desk
Thursday, August 25, 2016
How did Nextera kit specifically generate hetero-products out of PCR reactions? Why do the products have two different adaptors on their two ends?
Suppression PCR
http://www.nature.com/nmeth/journal/v6/n11/pdf/nmeth.f.272.pdf
http://www.nature.com/nmeth/journal/v6/n11/pdf/nmeth.f.272.pdf
Tuesday, August 23, 2016
[Solved] MacBook doesn't recognize Kindle
You might be using a charging-only cable instead of a data cable.
reference: https://discussions.apple.com/thread/6691745?tstart=0
reference: https://discussions.apple.com/thread/6691745?tstart=0
Monday, August 22, 2016
[兔兔助手] tutuapp new update: where is the newer version of Pokemon GO 懒人版
1. Download 兔兔助手正版
2. download from 兔兔助手 the app "Alfa Tournament"
Enjoy!
--------------
Now it's switched back , 20160904
2. download from 兔兔助手 the app "Alfa Tournament"
Enjoy!
--------------
Now it's switched back , 20160904
Saturday, August 20, 2016
[Pokemon GO] Did I catch something rare? Pokemon rarity distribution and frequency counts
Everyone knows that a pidgey can be found everywhere while a charizard is not so common. But what's a more quantitative way to see how lucky you are when you see, say, an Abra or Haunter?
Here are the big data results (averaging all the places over the world, including coastal and non-coastal):
1. Overall, the rarity distribution is very uneven:
1.1 You catch a pidgey for every eight pokemons you come across
1.2 The top 9 abundant pokemons make up half of all the pokemons you see. In other words, half of your effort is actually dealing with the 9 most annoying pokemons you're tired of.
2. These are the detailed percentages:
Based on their rarity, I divide them into 6 levels, with the most abundant group as Level 1 and the rarest group as Level 6. Below are the charts:
Level 6: those off the list (because our monitor cannot detect them frequent enough to estimate their probability)
Here is the full list with percentages of occurrence among all the pokemons. Ctrl + F / Command + F to find your favorite pokemon's score!
Here are the big data results (averaging all the places over the world, including coastal and non-coastal):
1. Overall, the rarity distribution is very uneven:
1.1 You catch a pidgey for every eight pokemons you come across
1.2 The top 9 abundant pokemons make up half of all the pokemons you see. In other words, half of your effort is actually dealing with the 9 most annoying pokemons you're tired of.
2. These are the detailed percentages:
Based on their rarity, I divide them into 6 levels, with the most abundant group as Level 1 and the rarest group as Level 6. Below are the charts:
Level 6: those off the list (because our monitor cannot detect them frequent enough to estimate their probability)
Here is the full list with percentages of occurrence among all the pokemons. Ctrl + F / Command + F to find your favorite pokemon's score!
Pidgey | 12.9% |
Rattata | 9.2% |
Zubat | 7.0% |
Magikarp | 5.5% |
Weedle | 4.8% |
Spearow | 4.3% |
Ekans | 2.78% |
Psyduck | 2.74% |
Drowzee | 2.61% |
Caterpie | 2.42% |
Goldeen | 2.13% |
Paras | 2.05% |
Poliwag | 2.04% |
Eevee | 2.03% |
NidoranF | 1.98% |
Krabby | 1.93% |
Staryu | 1.62% |
NidoranM | 1.61% |
Venonat | 1.55% |
Meowth | 1.27% |
Mankey | 1.19% |
Tentacool | 1.13% |
Geodude | 1.11% |
Horsea | 1.11% |
Growlithe | 1.09% |
Sandshrew | 1.06% |
Clefairy | 1.04% |
Slowpoke | 0.886% |
Bellsprout | 0.830% |
Exeggcute | 0.806% |
Pinsir | 0.797% |
Oddish | 0.778% |
Pidgeotto | 0.774% |
Gastly | 0.736% |
Squirtle | 0.731% |
Magnemite | 0.652% |
Bulbasaur | 0.642% |
Cubone | 0.595% |
Rhyhorn | 0.586% |
Machop | 0.567% |
Shellder | 0.525% |
Golbat | 0.516% |
Voltorb | 0.511% |
Jigglypuff | 0.474% |
Doduo | 0.464% |
Diglett | 0.445% |
Abra | 0.431% |
Ponyta | 0.389% |
Jynx | 0.384% |
Dratini | 0.366% |
Kakuna | 0.328% |
Raticate | 0.295% |
Pikachu | 0.286% |
Tauros | 0.281% |
Charmander | 0.277% |
Seel | 0.267% |
Scyther | 0.239% |
Vulpix | 0.230% |
Koffing | 0.192% |
Metapod | 0.183% |
Tangela | 0.178% |
Omanyte | 0.164% |
Dodrio | 0.155% |
Poliwhirl | 0.136% |
Kabuto | 0.131% |
Arbok | 0.122% |
Onix | 0.122% |
Electabuzz | 0.117% |
Magmar | 0.117% |
Fearow | 0.113% |
Tentacruel | 0.108% |
Hypno | 0.103% |
Pidgeot | 0.098% |
Sandslash | 0.094% |
Golduck | 0.089% |
Nidorina | 0.080% |
Seaking | 0.070% |
Weepinbell | 0.070% |
Gloom | 0.066% |
Kingler | 0.066% |
Venomoth | 0.066% |
Graveler | 0.061% |
Nidorino | 0.061% |
Grimer | 0.052% |
Ivysaur | 0.052% |
Parasect | 0.047% |
Primeape | 0.047% |
Slowbro | 0.047% |
Seadra | 0.038% |
Arcanine | 0.033% |
Beedrill | 0.033% |
Butterfree | 0.033% |
Dragonair | 0.033% |
Kadabra | 0.033% |
Cloyster | 0.028% |
Haunter | 0.028% |
Hitmonlee | 0.028% |
Marowak | 0.028% |
Nidoking | 0.023% |
Persian | 0.023% |
Victreebel | 0.023% |
Wartortle | 0.023% |
Dewgong | 0.019% |
Electrode | 0.019% |
Flareon | 0.019% |
Hitmonchan | 0.019% |
Porygon | 0.019% |
Rhydon | 0.019% |
Snorlax | 0.019% |
Gengar | 0.014% |
Lickitung | 0.014% |
Magneton | 0.014% |
Mr.Mime | 0.014% |
Poliwrath | 0.014% |
Vaporeon | 0.014% |
Weezing | 0.014% |
Aerodactyl | 0.009% |
Charmeleon | 0.009% |
Clefable | 0.009% |
Dugtrio | 0.009% |
Golem | 0.009% |
Jolteon | 0.009% |
Lapras | 0.009% |
Rapidash | 0.009% |
Starmie | 0.009% |
Venusaur | 0.009% |
Wigglytuff | 0.009% |
Alakazam | 0.005% |
Exeggutor | 0.005% |
Farfetch'd | 0.005% |
Gyarados | 0.005% |
Ninetales | 0.005% |
Omastar | 0.005% |
Vileplume | 0.005% |
Tuesday, August 16, 2016
[solved] mysterious payment/credit card fraud from showpmt.com. what's that charge and how to cancel
If you noticed that your credit card activity history has something mentioning showpmt.com like these people, you'd be careful:
I was charged a total of $39.99 from my checking account for no apparent reason by CHECKCARD 02/11 SHOWPMT.COM 8772151910
I was charged a total of $39.99 from my checking account for no apparent reason by CHECKCARD 02/11 SHOWPMT.COM 8772151910
posted 02/12/2014 by Joshua Montecino
Usually the payment is under $100 but still it's a fraud, and a possible cause is you registered for some membership and provided them with your credit card information including the three digit number you have on the back of your card.
Solution:
1.Go to Showpmt.com
2.click membership ( manage your membership)
3. type your partial credit card information and cancel
Usually these should be enough
But to be safe you'd better request a replacement of your credit card by calling your credit card company
Tricks you should learn:
When asked for credit card information, never provide full information. If they ask you for full information, be alerted, and provide WRONG expire data or 3-digit code. If they are not legal chargers, they wouldn't know that your information provided was wrong. (I've tested some websites' fraud credit card signup by typing incorrect expire date. Registration was successful and my credit card was not charged.)
Take care
Tuesday, August 9, 2016
[兔兔助手tutuapp][solved] unverified app
Previously you could just follow the steps described here:
http://troubleshoo.blogspot.com/2016/08/ios9-untrusted-enterprise-developer.html
But 兔兔助手changed their company!!!
So you basically have to reinstall and retrust. That's it
http://troubleshoo.blogspot.com/2016/08/ios9-untrusted-enterprise-developer.html
But 兔兔助手changed their company!!!
So you basically have to reinstall and retrust. That's it
Monday, August 8, 2016
Illumina Sequencing: Single indexing v.s. Dual indexing
http://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/indexed-sequencing-overview-guide-15057455-02.pdf
[Solved]Spotify cannot install on windows 8
1. Download the newest version of spotify here (if you are in the US) https://www.spotify.com/us/download/
2. press alt + ctrl + delete to open task manager
3. there should be two processes running by spotify. End them two by clicking on them and end task
4. Now go to folder C:\Users\Brian\AppData\Roaming
and delete the Spotify folder to remove data
5. Install SpotifySetup.exe as usual
2. press alt + ctrl + delete to open task manager
3. there should be two processes running by spotify. End them two by clicking on them and end task
4. Now go to folder C:\Users\Brian\AppData\Roaming
and delete the Spotify folder to remove data
5. Install SpotifySetup.exe as usual
how to check your current ios version
settings -> general -> about
Scroll down and see "version"
Saturday, August 6, 2016
[solved][Pokemon GO] where to find Mr. Mime, Kangaskhan and FARFETCH'D
Mr. Mime's home town is Madrid, Spain (Not London nor Paris nor Barcelona)
Kangaskhan is, of course, Australian, probably from Woollahra
Farfetch'd comes from Tsim Sha Tsui, Hong Kong
Kangaskhan is, of course, Australian, probably from Woollahra
Farfetch'd comes from Tsim Sha Tsui, Hong Kong
[Solved][Pokemon GO 懶人版] if you can't move and "GPS signal not found"
You can't move, you can see pokemons and you see the red bar with error messages "GPS signal not found", one solution is:
Turn off the wifi and use 4G or LTE
Turn off the wifi and use 4G or LTE
[Solved][Pokemon GO] No pokemons showing up, gyms and pokestops shown but not functional
If no pokemons are showing up where they should according to Skiplagged/Pokevision, and you can't flip any blue pokestops although you see them, one solution is:
Turn on the wifi if there is
Turn on the wifi if there is
Friday, August 5, 2016
[solved][pokemon GO] no poke stops or gyms or pokemons shown
The solution that worked for me:
restart the app again and again.
[Solved][Pokemon GO]our servers are humbled by your incredible response
My solution:
I turned off my wifi and started using LTE and it worked on my iphone5. It worked multiple times.
Apparently this error message is not necessarily related to their servers.
I turned off my wifi and started using LTE and it worked on my iphone5. It worked multiple times.
Apparently this error message is not necessarily related to their servers.
Tuesday, August 2, 2016
iOS9 Untrusted Enterprise Developer with no option to trust
Take pokemon GO hacked version as an example:
In iOS 9.1 and lower, go to Settings - General - Profiles - Your app source/Carey London Limited - tap on Trust button.
In iOS 9.2+, go to Settings - General - Device Management - Your app source/Carey London Limited - tap on Trust button.
reference: http://stackoverflow.com/questions/30722883/ios9-untrusted-enterprise-developer-with-no-option-to-trust
reference: http://stackoverflow.com/questions/30722883/ios9-untrusted-enterprise-developer-with-no-option-to-trust
Monday, August 1, 2016
[chase freedom]how to verify receipt of replacement credit card
1. go to chase.com/verify card
2. log in
3. click the verify button
2. log in
3. click the verify button
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