[Wed Oct 5 20:55:25 2011] Preparing output location ./tophat_out/
[Wed Oct 5 20:55:25 2011] Checking for Bowtie index files
[Wed Oct 5 20:55:25 2011] Checking for reference FASTA file
[Wed Oct 5 20:55:25 2011] Checking for Bowtie
Bowtie version: 0.12.5.0
[Wed Oct 5 20:55:25 2011] Checking for Samtools
Samtools Version: 0.1.17
[Wed Oct 5 20:55:25 2011] Checking reads
min read length: 75bp, max read length: 75bp
format: fastq
quality scale: phred33 (default)
Error locating program: prep_reads
I solved this problem simply by using the /path/to/install/bin/ as the current directory and putting all the excutables into this directory and run the data on this very directory!!! sounds stupid but it worked!!!
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