Thursday, November 5, 2015

ImageJ merge GFP and white field photos

Drag the two images to ImageJ to open;
Image -> color -> merge channels
Add GFP to green and white to gray.

Ctrl + shift + Z and Ctrl + shift+C to further adjust


One trick:  For GFP signal, you'd better use G4.5 method of scaling to make the weak signals visible

No comments:

Post a Comment